To determine the effect of space allowance during transport, a well as, the effect of varying length of lairage part way through transport were examined by measuring stress levels, injury, incidence of dehydration, effect of food restriction and rest of young calves during and following transportation. The calves were transported twice for nine hours, with a 1 or 12h lairage period between the two trips. The space allowances during transport were either 0.375 or 0.475 m2 per calf. The control calves, which were not transported, were fed milk replacer and were also provided with water either at the normal times or only when the calves were given these fluids during transport. Calves that were transported spent significantly less time lying down and had significantly higher cortisol concentrations than the control calves. Increasing the amount of space available per calf did not result in an increased incidence of injuries and or the calves were still able to keep their balance. A lairage period of one hour provided enough time to feed the calves milk replacer, but did not given the calves much of an opportunity to rest. The duration of the lairage period midway through transportation did not have an effect on any of the measurements taken and the shorter lairage time did not appear to negatively affect welfare. Even though there was not any difference in the immune system measurements between the transport and control calves, transportation appeared to negatively affect the health status of the calves.