The reactivity of ewes to their lambs and to humans after lambing was examined. Six weeks prior to lambing, the ewes were repeated exposed to several stressors that varied in intensity. The ewes were stressed by transporting them alone, isolating them or isolating them with a dog. The ewes were underwent a stressful session twice a week. Each session last one hour, and in total, each ewe underwent 10 sessions. The control ewes were not disturbed. Blood samples were collected during the first, fifth and ninth stress sessions, in order to measure their cortisol response. The ewe’s maternal reactivity and fear of humans was measured in seven scenarios. One hour after birth, a human approached the lambing site and the lamb. Five minutes after tagging someone held the lamb. Six hours after birth the ewe was forced across a hallway to reach her lambs. Six hours after birth, in order for the ewe to remain close to her lamb, she was forced to tolerate the presence of a human. The ewe was also separated from the lamb and forced to enter the lamb box. One month following birth, a human approached the ewe, and then measured the flight distance of the ewe. The highest cortisol response was measured in ewes that were transported, during the first and ninth sessions. The ewes did demonstrate habituation to every treatment over time. During the tests conducted one and six hours after birth, ewes that were isolated attempted to maintain contact with their lambs, while the ewes that were transported did not. The control ewes response was moderate compared to the transport and isolation treatment ewes. One month after birth, the isolated ewes continued to show less avoidance of humans than transported ewes, while the control ewes remaining intermediate between the responses of the other two treatments. There were also correlation found between the cortisol response during the stress and the responses made 1 hour, 6 hours and 1 month after birth. The variation in the ewes’ behaviour after lambing was likely due to the different stressors causing varying degrees of fear towards humans. The behaviour is not related to the ewe developing a weaker mother-young bond. Hence, repeated isolation reduced the ewe’s fear of humans, while repeated isolated transportation increases a ewe’s fear of humans.