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Author(s): M. Wilson
Publication Date: April 5, 2006
Reference: London Swine Conference, 2006
Country: Canada

Summary:

Gender pre-selected sperm that have been sorted using flow cytometric sorting are becoming widely used in the cattle industry, but the swine industry has been slower to adopt the technology. This is partially because swine require a greater number of spermatozoa for fertilization. In early gender selection research, physical and mechanical methods have been shown to be largely inefficient. Several machines using flow cytometry sorting have recently been shown to be effective by confirmation with fluorescent staining, counting microsatellite DNA probe, and PCR. They have also proved efficient at gender selection through producing in vivo and in vitro litters. Gender selection is not yet commercially viable due to the special insemination techniques needed (AI requires too many sperm), and further developments needed for sperm sorters.

Embryonic transfer has successfully been done with swine, but is not widely used due to the necessary surgical procedures used to retrieve and sometimes place the embryos. Non-surgical embryo placement has been attempted for decades, and usually have low farrowing rates and litter sizes. However, farrowing rates of up to 70% have been achieved when a Spirette and inner catheter are used. Non-surgical embryo placement requires specific training, although new technology may make it a more viable option in the future. Cloning is another aspect of reproductive technology that may be more widely utilized in the future. Cloning requires an excellent embryonic source, precise procedures, and a sterile environment. Cloning may be used to recreate top performing animals, for use in experimental models, to genetically modify genes for specific traits, and for health-care related applications.

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