Significant spontaneous fetal loss of unknown cause occurs in North\u00a0American commercial swine. About 30% of conceptuses, thought to be\u00a0genetically normal, are lost during the peri-attachment period. An additional\u00a020% are lost at mid-pregnancy. Littermate endometrial and trophoblast\u00a0biopsies were studied by quantitative real-time PCR for gene\u00a0expression, and immunohistochemistry for protein expression at gestation\u00a0day (gd)15\u201323 and 50. RNA analyses were also conducted on endometrial\u00a0lymphocytes and arterial endothelial cells removed from biopsies\u00a0by laser capture microdissection. Genes were selected for study from\u00a0human literature and cloned as required. As in humans, angiogenic,\u00a0cytokine, chemokine and chemokine decoy receptor gene expression\u00a0occurs at the porcine maternal\u2013fetal interface. In each tissue studied,\u00a0distinct patterns of expression are found between early and mid-pregnancy,\u00a0as well as between viable and arresting conceptus attachment\u00a0sites. These changes involve both endometrial lymphocytes and dendritic\u00a0cells. Restriction in endometrial angiogenesis, reduction in expression\u00a0of the chemokine decoy receptor D6, and reduction in dendritic
\ncell numbers contribute to fetal arrest. In peri-attachment loss, interferon-\u00a0c is more abundantly transcribed than tumor necrosis factor-a,\u00a0but this ratio is reversed during midgestation failure. Further characterization\u00a0of spontaneous fetal loss in pigs will identify targets for modification\u00a0by hog producers and may provide a model for identification of\u00a0antecedents to fetal loss in humans.<\/p>\n
Our studies also characterize spontaneous fetal loss\u00a0in pigs as an excellent animal model to assist in\u00a0understanding the maternal\u2013fetal interactions leading\u00a0to pregnancy failure in humans. We have found\u00a0porcine and human endometria, as well as trophoblasts,\u00a0express similar cytokines, chemokines, and\u00a0chemokine decoy receptors. The large amount of\u00a0material available for study from porcine attachment\u00a0sites, the reliability of spontaneous fetal arrest and\u00a0its consistency of timing, the accessibility to timed\u00a0pregnant reproductive tracts, and the ability to conduct\u00a0paired sampling of littermates with different\u00a0survival potential are all strong features that should\u00a0complement studies in patients where these features\u00a0are limiting.<\/p>\n","protected":false},"excerpt":{"rendered":"
Significant spontaneous fetal loss of unknown cause occurs in North\u00a0American commercial swine. About 30% of conceptuses, thought to be\u00a0genetically normal, are lost during the peri-attachment period. An additional\u00a020% are lost at mid-pregnancy. Littermate endometrial and trophoblast\u00a0biopsies were studied by quantitative real-time PCR for gene\u00a0expression, and immunohistochemistry for protein expression at gestation\u00a0day (gd)15\u201323 and 50. RNA […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[19,930],"tags":[25535,4685,640,3804,102,14,35],"class_list":["post-14794","post","type-post","status-publish","format-standard","hentry","category-pork-insight-articles","category-production","tag-a-review-of-molecular-contrasts-between-arresting-and-viable-porcine-attachment-sites","tag-access","tag-animal","tag-genetic","tag-gestation","tag-pig","tag-swine"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14794","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=14794"}],"version-history":[{"count":2,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14794\/revisions"}],"predecessor-version":[{"id":14797,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14794\/revisions\/14797"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=14794"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=14794"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=14794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}