This study was conducted to examine antimicrobial resistances, plasmid profiles, and pulsed-field gel electrophoresis patterns of\u00a080 Salmonella Typhimurium DT104 strains (including DT104a and DT104b) recovered from pig and\u00a0environmental fecal samples on 17 swine farms in Ontario. No resistance was observed to amoxicillin\/clavulanic acid, apramycin,\u00a0carbadox, cephalothin, ceftriaxone, ceftiofur, cefoxitin, ciprofloxacin, nalidixic acid, trimethoprim, and tobramycin.<\/p>\n
However,\u00a0the isolates exhibited resistance against 4 to 10 antimicrobials with the most frequent resistance being to sulfonamides (Su),\u00a0ampicillin (A), streptomycin (S), spectinomycin (Sp), chloramphenicol (C), tetracycline (T), and florfenicol (F).\u00a0It was found that 88% of isolates shared thetypical R-type \u201cACSSuT,\u201d which has been frequently reported in \u00a0association with DT104 isolates from different sources in Canada and other countries. Resistances to \u201cACT,\u201d \u201cACNT,\u201d and\u00a0\u201cACNSSuTm\u201d were the most common patterns that occurred among\u00a0DT104 isolates recovered from healthy pigs in the slaughterhouses\u00a0receiving animals from Quebec, Ontario, Manitoba, Saskatchewan,\u00a0and British Columbia.<\/p>\n
The isolates were classified into 23 distinct\u00a0genotypes by PFGE-SpeI 1 BlnI when difference in at least one fragment was defined as a distinct genotype. In total, 39 distinct\u00a0\u201ctypes\u201d were observed when defining a \u201ctype\u201d based on the combination of antimicrobial resistance, plasmid pattern, and\u00a0PFGE-SpeI 1 BlnI for each isolate. The highest diversity was 0.96 (95% CI: 0.92, 0.96) for the \u201ctype\u201d described above followed by\u00a00.92 (95% CI: 0.88, 0.93) for PFGE-SpeI 1 BlnI. The diversity of DT104 isolates indicates there might be multiple sources for this\u00a0microorganism on swine farms. This knowledge might be used to track these sources, as well as to study the extent of human\u00a0salmonellosis attributed to pork compared to food products derived from other food-producing animals<\/p>\n","protected":false},"excerpt":{"rendered":"
This study was conducted to examine antimicrobial resistances, plasmid profiles, and pulsed-field gel electrophoresis patterns of\u00a080 Salmonella Typhimurium DT104 strains (including DT104a and DT104b) recovered from pig and\u00a0environmental fecal samples on 17 swine farms in Ontario. No resistance was observed to amoxicillin\/clavulanic acid, apramycin,\u00a0carbadox, cephalothin, ceftriaxone, ceftiofur, cefoxitin, ciprofloxacin, nalidixic acid, trimethoprim, and tobramycin. However,\u00a0the […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[1809,640,2771,25732,14,2774,35,18011],"class_list":["post-14947","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-acid","tag-animal","tag-iron","tag-nonruminant-nutrition-symposium-understanding-protein-synthesis-and-degradation-and-their-pathway-regulations","tag-pig","tag-pulse","tag-swine","tag-tot"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14947","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=14947"}],"version-history":[{"count":1,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14947\/revisions"}],"predecessor-version":[{"id":14949,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14947\/revisions\/14949"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=14947"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=14947"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=14947"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}