Prenatal mortality reaching 30% occurs during the first weeks of gestation in commercial swine. Mechanisms for this are unknown\u00a0although poor uterine blood supply has been postulated. In other species, vascular endothelial growth factor, hypoxia-inducible\u00a0factor 1-\u0001, and IFN-\u0002 regulate gestational endometrial angiogenesis. Vascular endothelial growth factor and hypoxia-inducible\u00a0factor 1-\u0001 are also important for placental angiogenesis while trophoblastic expression of Fas ligand is thought to protect conceptuses\u00a0against immune-mediated pregnancy loss. In this study, we document dynamic, peri-implantation differences in transcription\u00a0of genes for angiogenesis, cytokine production, and apoptosis regulation in the endometrium, and laser capture microdissected\u00a0endometrial lymphocytes and trophoblasts associated with healthy or viable but arresting porcine fetuses. In healthy\u00a0implantation sites, endometrial gene expression levels differed between anatomic subregions and endometrial lymphocytes showed\u00a0much greater transcription of angiogenic genes than trophoblasts. In arresting fetal sites, uterine lymphocytes had no angiogenic\u00a0gene transcription and showed rapid elevation in transcription of proinflammatory cytokines Fas and Fas ligand while trophoblasts\u00a0showed elevated transcription of IFN-\u0002 and Fas. This model of experimentally accessible spontaneous fetal loss, involving\u00a0blocked maternal angiogenesis, should prove valuable for further investigations of peri-implantation failure of normally conceived\u00a0and surgically transferred embryos in many species, including the human<\/p>\n","protected":false},"excerpt":{"rendered":"
Prenatal mortality reaching 30% occurs during the first weeks of gestation in commercial swine. Mechanisms for this are unknown\u00a0although poor uterine blood supply has been postulated. In other species, vascular endothelial growth factor, hypoxia-inducible\u00a0factor 1-\u0001, and IFN-\u0002 regulate gestational endometrial angiogenesis. Vascular endothelial growth factor and hypoxia-inducible\u00a0factor 1-\u0001 are also important for placental angiogenesis while […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[4685,35],"class_list":["post-15131","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-access","tag-swine"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15131","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=15131"}],"version-history":[{"count":2,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15131\/revisions"}],"predecessor-version":[{"id":15134,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15131\/revisions\/15134"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=15131"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=15131"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=15131"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}