{"id":3686,"date":"2006-01-01T01:01:01","date_gmt":"2006-01-01T01:01:01","guid":{"rendered":"http:\/\/export.maxmaziy.php.nixsolutions.com\/?p=3686"},"modified":"2006-01-01T01:01:01","modified_gmt":"2006-01-01T01:01:01","slug":"microbial-source-tracking-using-host-specific-fame-profiles-of-fecal-coliforms","status":"publish","type":"post","link":"http:\/\/prairieswine.com\/rsc\/microbial-source-tracking-using-host-specific-fame-profiles-of-fecal-coliforms\/","title":{"rendered":"Microbial source tracking using host specific FAME profiles of fecal coliforms"},"content":{"rendered":"<p>The objective of this study was to investigate the host-specific differences in fatty acid<br \/>\nmethyl ester (FAME) profiles of fecal coliforms (FC). A known-source library was<br \/>\nconstructed with 314 FC isolates cultured from 6 possible sources of fecal pollution; 99<br \/>\nisolates from sewage; 29 from bovine; 29 from poultry; 50 from swine; 46 from waterfowl;<br \/>\nand 61 from deer. It was found that the hydroxy FAMEs 12:0 2OH, 12:0 3OH, and 14:0 2OH<br \/>\nwere exclusively associated with isolates of human origin. On the other hand, 3 saturated<br \/>\nFAMEs, 10:0, 15:0, and 18:0 were found only in isolates from non-human sources, 15:0 being<br \/>\nassociated with livestock samples only. In addition to the presence of these signature<br \/>\nFAMEs, the mean relative masses of 16:1 o7c and 16:1 ISO\/14:0 3OH were significantly<br \/>\ndifferent between the isolates of human and non-human origins. A linear discriminant<br \/>\nfunction differentiated FC isolates of human origin from those of livestock and wildlife<br \/>\norigin at 99% accuracy. These results strongly suggest that the FAME profiles of FC show<br \/>\nstatistically significant host specificity and may have the potential to be used as a<br \/>\nphenotypic microbial source tracking tool.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The objective of this study was to investigate the host-specific differences in fatty acid methyl ester (FAME) profiles of fecal coliforms (FC). A known-source library was constructed with 314 FC isolates cultured from 6 possible sources of fecal pollution; 99 isolates from sewage; 29 from bovine; 29 from poultry; 50 from swine; 46 from waterfowl; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[930],"tags":[1809,8882,13816,22399,2210,19644,5594,2851,5595,3005,11297,4037,10859,22808,16262,424,138,1309,35,20911,69,15479],"class_list":["post-3686","post","type-post","status-publish","format-standard","hentry","category-production","tag-acid","tag-al","tag-ass","tag-coli","tag-coliforms","tag-dd","tag-fame","tag-fat","tag-fecal","tag-host","tag-masse","tag-microbial","tag-nat","tag-pic","tag-pl","tag-pollution","tag-poultry","tag-sew","tag-swine","tag-t","tag-water","tag-wild"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/3686","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=3686"}],"version-history":[{"count":0,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/3686\/revisions"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=3686"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=3686"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=3686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}