For artificial insemination (AI) in pigs, preservation of liquid boar semen at
\n16\u201320 \u00b0C is still common practice as sperm cryopreservation remains suboptimal
\nin this species. To meet the different needs of the swine industry, several
\nextenders have been developed to preserve semen in liquid form for short- and
\nlong-term storage. In the present study, three different commercial extenders
\ndevised for short-term (BTS+) or long-term preservation (MR-A and X-Cell),
\nwere used to test whether storage of semen from four mature, fertile boars at
\n17 \u0002C for 96 h would affect sperm characteristics relevant for fertility, such as
\nmotility, membrane integrity and chromatin stability. Computer-assisted sperm
\nanalysis, and stainings with the acylated membrane dye SYBR-14\/propidium
\niodide, and acridine orange in connection with flow cytometry were used to
\nevaluate these variables. Percentages of total motile spermatozoa decreased
\nslightly, but significantly, after 72\u201396 h. While membrane integrity values varied
\nduring the period of study, no significant changes in either membrane
\nintegrity or chromatin stability were, however, registered. This suggests a customary
\n96-day storage at 17 \u00b0C in these extenders was too short an interval to
\ncause losses of integrity in nuclear DNA in the boar population studied.<\/p>\n","protected":false},"excerpt":{"rendered":"
For artificial insemination (AI) in pigs, preservation of liquid boar semen at 16\u201320 \u00b0C is still common practice as sperm cryopreservation remains suboptimal in this species. To meet the different needs of the swine industry, several extenders have been developed to preserve semen in liquid form for short- and long-term storage. In the present study, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[930],"tags":[8882,13816,5162,23964,21246,785,5621,3734,286,25122,20007,5620,5381,5622,10859,7545,15459,14,33,16262,92,24510,3753,4467,5623,309,35,16004,20911,1579,18011,6053],"class_list":["post-3941","post","type-post","status-publish","format-standard","hentry","category-production","tag-al","tag-ass","tag-boar","tag-boar-fertility","tag-boar-semen","tag-boars","tag-chromatin-stability","tag-dna","tag-dust","tag-eet","tag-gh","tag-insemination","tag-light","tag-liquid-semen","tag-nat","tag-optimal","tag-ped","tag-pig","tag-pigs","tag-pl","tag-plasma","tag-register","tag-semen","tag-species","tag-sperm-viability","tag-storage","tag-swine","tag-swine-industry","tag-t","tag-test","tag-tot","tag-use"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/3941","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=3941"}],"version-history":[{"count":0,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/3941\/revisions"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=3941"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=3941"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=3941"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}