One hundred Hampshire X Duroc crossbred
\npigs and 100 Nebraska Index line pigs were infected
\nwith porcine reproductive and respiratory syndrome virus
\n(PRRSV) and evaluated for resistance and susceptibility.
\nControls (100\/line) were uninfected littermates
\nto infected pigs. Viremia (V), BW change (WT\u0192\u00a2), and
\nrectal temperature at 0, 4, 7, and 14 d postinfection
\nwere recorded. Lung, bronchial lymph node (BLN), and
\nblood tissue were collected at necropsy (14 d postinfection).
\nInfected pigs were classified as low or high responders
\nto PRRSV based on the first principal component
\nfrom principal component analyses of all variables.
\nLow responders to PRRSV (low PRRSV burden) and
\ntheir uninfected littermates were assigned to the low
\n(L) class. High responders to PRRSV (high PRRSV
\nburden) and their uninfected littermates were assigned
\nto the high (H) class. Infected pigs in the L class had
\nlarge WT\u0192\u00a2, low V, and few lung lesions; H-class pigs
\nhad small WT\u0192\u00a2, high V, and many lung lesions. Ribonucleic
\nacid was extracted from lung and BLN tissue of
\nthe 7 highest and 7 lowest responders per line and from
\neach of their control littermates. A control reference
\ndesign was used, and cDNA from each reference sample
\ntissue was prepared from pooled RNA extracted from
\n2 control pigs from each line whose infected littermates
\nhad a principal component value of 0. Design variables
\nin data analyses were line (Index vs. Hampshire X Duroc),
\nclass (H vs. L), treatment (infected vs. uninfected
\ncontrols), and slide\/pig as error. Oligo differential expression
\nwas based on P < 0.01 occurring in both lung\nand BLN. Line and treatment effects were significant\nfor 38 and 541 oligos, respectively, in both lung and\nBLN. Line ~ class interaction existed for expression\nof thymosin \u0192\u00c0-4, DEAD box RNA helicase 3, acetylcholinesterase,\nand Homo sapiens X (inactive)-specific\ntranscript in both tissues. Treatment ~ class existed\nfor expression of CCAAT\/enhancer-binding \u0192\u00c2 protein,\nnuclear factor of \u0192\u00c8 light polypeptide gene enhancer in B\ncells inhibitor \u0192\u00bf, thioredoxin-interacting protein, major\nfacilitator superfamily domain containing 1, and unknown\nsequences SS00012040 and SS00012343. Line ~\ntreatment and line ~ treatment ~ class interactions\nwere not significant. Possible important genetic associations\nfor fine-mapping candidate genes related to\nresponse to PRRSV and determining causative alleles\nwere revealed.\n<\/p>\n","protected":false},"excerpt":{"rendered":"
One hundred Hampshire X Duroc crossbred pigs and 100 Nebraska Index line pigs were infected with porcine reproductive and respiratory syndrome virus (PRRSV) and evaluated for resistance and susceptibility. Controls (100\/line) were uninfected littermates to infected pigs. Viremia (V), BW change (WT\u0192\u00a2), and rectal temperature at 0, 4, 7, and 14 d postinfection were recorded. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[930],"tags":[1809,8882,13816,2854,21480,2817,3734,2559,6742,3804,20007,9459,26380,5381,818,8791,6766,14,33,16262,19940,6767,17743,4181,5303,8926,22252,20911,4874,99,52,24994],"class_list":["post-4065","post","type-post","status-publish","format-standard","hentry","category-production","tag-acid","tag-al","tag-ass","tag-bin","tag-choline","tag-cla","tag-dna","tag-duroc","tag-gene-expression","tag-genetic","tag-gh","tag-hampshire","tag-infec","tag-light","tag-litter","tag-map","tag-microarray","tag-pig","tag-pigs","tag-pl","tag-porc","tag-porcine-reproductive-and-respiratory-syndrome-virus-resistance","tag-prod","tag-prrs","tag-prrsv","tag-psy","tag-rectal-temp","tag-t","tag-tan","tag-temperature","tag-treatment","tag-usa"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/4065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=4065"}],"version-history":[{"count":0,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/4065\/revisions"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=4065"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=4065"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=4065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}