To identify DNA markers associated
\nwith performance, carcass, and meat production traits
\nincluding muscle postmortem cathepsin activity, several
\nporcine genes encoding for lysosomal proteinases
\n(cathepsin B, CTSB; cathepsin D, CTSD; cathepsin F,
\nCTSF; cathepsin H, CTSH; cathepsin L, CTSL; and
\ncathepsin Z, CTSZ) and for a cathepsin inhibitor (cystatin
\nB) were investigated. Single nucleotide polymorphisms
\nwere identified in CTSD, CTSH, CTSL, and
\nCTSZ genes with a combination of in silico expressed
\nsequence tag database mining and single-strand conformation
\npolymorphism analysis. Sequencing and PCRRFLP
\nprotocols were used to validate the identified
\npolymorphisms. Allele frequencies at these loci were
\ninvestigated in Italian Large White, Landrace, Duroc,
\nPi\u00e9train, Belgian Landrace, Hampshire, and Meishan
\nbreeds. Genotyping CTSD and CTSH markers made it
\npossible to genetically map these genes to SSC 2 and
\n7, respectively. Markers in CTSD, CTSH, CTSL, and
\nCTSZ genes, together with mutations we previously reported
\nin cystatin B, CTSB, and CTSF genes, were
\ngenotyped in an Italian Large White sib-tested population
\n(272 or 482 animals). For these animals, meat
\nquality traits (cathepsin B activity, pH measured at 2 h
\npostmortem, pH measured at 24 h postmortem, glycogen,
\nlactate, and glycolytic potential of semimembranosus
\nmuscle) and EBV for ADG, lean cuts (LC), backfat
\nthickness (BFT), ham weight (HW), and feed:gain ratio
\n(FGR) were determined. Analyzed markers did not
\nshow any association with muscle cathepsin B activity.
\nThus, it could be possible that different genes, other
\nthan these investigated candidates, affect this trait,
\nwhich is correlated with the excessive softness defect
\nof dry-cured hams. The results of association analysis
\nconfirmed the effects we already reported in another
\nstudy for CTSF on ADG (P = 0.008), LC (P = 0.001),
\nand BFT (P = 0.02). Moreover, CTSD was associated
\nwith ADG, LC (P < 0.0001), BFT, HW, and FGR (P\n< 0.001); CTSH was associated with FGR (P = 0.026);\nand CTSZ was associated with ADG (P = 0.006), LC\n(P = 0.01), HW (P = 0.024), and FGR (P = 0.029).\nThe biochemical and physiological functions of the lysosomal\nproteinases, together with the results obtained\nin our investigation, suggest that the cathepsin gene\nfamily might play important roles affecting economic\ntraits in pigs.\n<\/p>\n","protected":false},"excerpt":{"rendered":"
To identify DNA markers associated with performance, carcass, and meat production traits including muscle postmortem cathepsin activity, several porcine genes encoding for lysosomal proteinases (cathepsin B, CTSB; cathepsin D, CTSD; cathepsin F, CTSF; cathepsin H, CTSH; cathepsin L, CTSL; and cathepsin Z, CTSZ) and for a cathepsin inhibitor (cystatin B) were investigated. Single nucleotide polymorphisms […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[930],"tags":[8882,640,10033,13816,2854,2349,7807,6768,6769,6770,6321,3734,20077,2559,2851,6904,46,3804,20007,158,24852,9459,7099,8791,10858,8306,94,2909,9230,10859,14784,15459,63,2560,19736,14,33,16262,19940,17743,27282,95,5269,20581,5743,25698,20911,4874,1579,26223,1301],"class_list":["post-4066","post","type-post","status-publish","format-standard","hentry","category-production","tag-al","tag-animal","tag-arc","tag-ass","tag-bin","tag-breed","tag-breeds","tag-candidate-gene","tag-cathepsin","tag-cystatin","tag-database","tag-dna","tag-dna-marker","tag-duroc","tag-fat","tag-fee","tag-feed","tag-genetic","tag-gh","tag-growth","tag-growth-promoters-in-pietrain","tag-hampshire","tag-landrace","tag-map","tag-mark","tag-markers","tag-meat","tag-meat-production","tag-meishan","tag-nat","tag-ness","tag-ped","tag-performance","tag-pietrain","tag-pietrain-swine","tag-pig","tag-pigs","tag-pl","tag-porc","tag-prod","tag-production","tag-quality","tag-report","tag-single-nucleotide-polymorphism-swine","tag-single-nucleotide-polymorphism","tag-swine-pietrain-german","tag-t","tag-tan","tag-test","tag-the-effects","tag-weight"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/4066","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=4066"}],"version-history":[{"count":0,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/4066\/revisions"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=4066"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=4066"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=4066"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}