Uterine and oviductal samples were collected from twenty two primiparous
\nsows on their second estrus following weaning. Based on the timing of
\novulation determined by real-time ultrasound, samples were collected from a
\nminimum of three sows whose temporal reproductive status corresponded to
\nthe germinal vesicle (GV) and metaphase two (MII) stages of oocyte
\nmaturation, and the 2-cell, 4-cell, early morula (8- to 16-cell), morula and
\nblastocyst stages of embryonic development. Each uterine horn was divided
\ninto three equal length sections by ligature to represent the lower- (cervical),
\nmid- and upper (oviductal) sections of the horn. Warm PBS was then used to
\nflush each oviduct (5ml) and each section of the uterine horns (20 m).
\nHyaluronic acid concentrations in flushings were determined using an HA
\nspecific ELISA (Hyaluronan Duo Set, R&D systems) and the volume of each
\nflush was used to estimate total HA content (ng per flush). No differences
\n(P>0.05) in HA content were found between uterine sections at any stage of
\ngestation. HA content of uterine flushings decreased (P<0.0001) between the\nGV and 2-cell stages, and then steadily increased (P<0.001-0.01) through the\nmorula and blastocyst stages of development.\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Uterine and oviductal samples were collected from twenty two primiparous sows on their second estrus following weaning. Based on the timing of ovulation determined by real-time ultrasound, samples were collected from a minimum of three sows whose temporal reproductive status corresponded to the germinal vesicle (GV) and metaphase two (MII) stages of oocyte maturation, and […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[930],"tags":[1809,8882,3965,7116,7495,102,20007,7496,7497,14,16262,17743,1902,104,96,8701,20911,18011,3864,7498,77],"class_list":["post-4693","post","type-post","status-publish","format-standard","hentry","category-production","tag-acid","tag-al","tag-content","tag-elisa","tag-flush","tag-gestation","tag-gh","tag-ha","tag-hyaluronic-acid","tag-pig","tag-pl","tag-prod","tag-reproduction","tag-sow","tag-sows","tag-swine-uterine-horn","tag-t","tag-tot","tag-ultrasound","tag-uterine-horn","tag-weaning"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/4693","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=4693"}],"version-history":[{"count":0,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/4693\/revisions"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=4693"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=4693"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=4693"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}