Post-weaning multisystemic wasting syndrome (PMWS) is a complex disease syndrome in
\nswine, affecting nursery and fattening pigs. Although ongoing evidence suggests that
\nporcine circovirus type-2 (PCV2) is the causal agent of PMWS, the host immune system
\nappears to have a crucial role in the PMWS pathogenesis of PCV2-affected pigs. Owing to
\ndifficulties in producing a biologically pure form of PCV2 devoid of the other viral agents
\ncommonly present in swine tissues, we decided to use a tandem-cloned PCV2 DNA
\nproviding highly pure grade reagent in order to monitor the virulence of PCV2 alone or
\nwith an immunostimulating co-factor, granulocyte-macrophage colony-stimulating factor
\n(GM-CSF). A single intramuscular injection of tandem-cloned PCV2 DNA into 5-weekold
\npiglets produced plasmid to viral genome progeny and infectious particles as early as 8
\ndays post-injection in all the organs tested (the lung, the tonsil and the inguinal, mesenteric,
\nbronchial and upper-right axial lymph nodes). The initial plasmid load was not
\ndetected with the help of primers designed to specifically detect the acceptor plasmid, thus
\nconfirming the replication of the viral genome. Despite the presence of a high level of
\nPCV2 genome copies in the lymphoid organs \u2013 the tonsil and the lung \u2013 and the presence
\nof infectious particles, no detectable clinical manifestations or pathological lesions were
\nobserved in the transfected pigs over the period of observation, regardless of whether they
\nhad been co-injected with plasmid containing GM-CSF DNA or had received plasmid
\ncontaining PCV2 DNA alone. GM-CSF encoding DNA injection had no significant effect
\non viral replication or on the production of viral particles and appearance of the disease.<\/p>\n","protected":false},"excerpt":{"rendered":"
Post-weaning multisystemic wasting syndrome (PMWS) is a complex disease syndrome in swine, affecting nursery and fattening pigs. Although ongoing evidence suggests that porcine circovirus type-2 (PCV2) is the causal agent of PMWS, the host immune system appears to have a crucial role in the PMWS pathogenesis of PCV2-affected pigs. Owing to difficulties in producing a […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[928],"tags":[13310,8882,6618,2899,8547,15264,24566,60,3734,2851,7904,20007,4823,4824,6492,3005,6577,5851,4825,26380,4826,4827,324,4951,7816,4828,3465,14,126,89,33,16262,2901,19940,6230,17743,27282,35,20911,4874,26635,1579,24994,6053,4829,77],"class_list":["post-5438","post","type-post","status-publish","format-standard","hentry","category-welfare","tag-infectious-dna-swine","tag-al","tag-articles","tag-circovirus","tag-complex","tag-csf","tag-cv2","tag-disease","tag-dna","tag-fat","tag-fattening","tag-gh","tag-gm-csf","tag-granulocyte-macrophage-colony-stimulating-factor","tag-help","tag-host","tag-immune","tag-immune-system","tag-in-vivo-transfection","tag-infec","tag-infectious-dna","tag-intramuscular-route","tag-nursery","tag-particle","tag-pcv","tag-pcv2","tag-pea","tag-pig","tag-piglet","tag-piglets","tag-pigs","tag-pl","tag-pmws","tag-porc","tag-porcine-circovirus","tag-prod","tag-production","tag-swine","tag-t","tag-tan","tag-temi","tag-test","tag-usa","tag-use","tag-wasting-syndrome","tag-weaning"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/5438","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=5438"}],"version-history":[{"count":0,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/5438\/revisions"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=5438"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=5438"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=5438"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}