{"id":6312,"date":"2010-09-02T15:17:50","date_gmt":"2010-09-02T15:17:50","guid":{"rendered":"http:\/\/prairieswine.com\/rsc\/?p=6312"},"modified":"2011-12-02T15:00:12","modified_gmt":"2011-12-02T21:00:12","slug":"development-of-rapid-immunoassays-for-the-detection-of-ractopamine-in-swine-urine-2","status":"publish","type":"post","link":"http:\/\/prairieswine.com\/rsc\/development-of-rapid-immunoassays-for-the-detection-of-ractopamine-in-swine-urine-2\/","title":{"rendered":"Development of rapid immunoassays for the detection of ractopamine in swine urine"},"content":{"rendered":"<p>The monoclonal antibodies (mAbs) against ractopamine (Rac) were prepared and their properties identified by indirect competitive enzyme-linked immunoabsorbant assay (ELISA). The IC50 of mAbs was 2.7 ng ml_1 towards Rac or 9.3 ng ml_1 towards Rac-glucuronides and no cross-reactivity (CR) towards other competitors except dobutamine (CR: 3.76%). Based on the mAbs, the Rac-kit (kit) and Rac-strip (strip) were developed to detect Rac residues in swine urine. The strip and kit assay could be performed within 5\u201310 min and 2 h, respectively, allowing the analysis of urine samples without the need for sample clean-up. The detection limits were 1 ngml_1 for kit and 3 ngml_1 with the unaided eye, and 0.2 ng ml_1 with the Strip Reader for strip. The correlation coefficients (R2) were 0.988 for kit in the range 0\u2013128.0 ng ml_1, and 0.987 for strip in the range 0\u201310.8 ng ml_1. Comparing the gas chromatography-mass spectrometry (GC-MS) with the kit or strip in swine urine spiked with Rac standards, the differences ranged from 1.4% to 4.5% for kit and 1.0% to 4.7% for strip. However, the differences were greater than 54% for the kit and 55% for the strip test for the analysis of urine from swine treated with Rac. The results obtained from GC-MS using hydrolysed urine samples were generally in good agreement with those obtained from strip or kit using non-hydrolysed urine samples.<\/p>\n<p>For more information the full article can be found at\u00a0 <a href=\"http:\/\/www.tandfonline.com\/loi\/tfac19\">http:\/\/www.tandfonline.com\/loi\/tfac19<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The monoclonal antibodies (mAbs) against ractopamine (Rac) were prepared and their properties identified by indirect competitive enzyme-linked immunoabsorbant assay (ELISA). The IC50 of mAbs was 2.7 ng ml_1 towards Rac or 9.3 ng ml_1 towards Rac-glucuronides and no cross-reactivity (CR) towards other competitors except dobutamine (CR: 3.76%). Based on the mAbs, the Rac-kit (kit) and [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[930],"tags":[8882,13816,3814,9693,23694,12670,1443,316,15459,16262,3871,8394,35,20911,4874,1579,1213,11771],"class_list":["post-6312","post","type-post","status-publish","format-standard","hentry","category-production","tag-al","tag-ass","tag-competitive","tag-development-of-rapid-immunoassays-for-the-detection-of-ractopamine-in-swine-urine","tag-dir","tag-down","tag-enzyme","tag-gas","tag-ped","tag-pl","tag-ractopamine","tag-ractopamine-in-swine","tag-swine","tag-t","tag-tan","tag-test","tag-urine","tag-www-prairieswine-com"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/6312","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=6312"}],"version-history":[{"count":7,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/6312\/revisions"}],"predecessor-version":[{"id":10706,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/6312\/revisions\/10706"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=6312"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=6312"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=6312"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}