{"id":8817,"date":"2011-08-24T15:38:33","date_gmt":"2011-08-24T15:38:33","guid":{"rendered":"http:\/\/prairieswine.com\/rsc\/?p=8817"},"modified":"2011-11-25T15:06:30","modified_gmt":"2011-11-25T15:06:30","slug":"electron-microscopy-finder-grids-link-quantitative-cytometry-with-high-resolution-confocal-imaging-in-the-study-of-tgf-%ce%b21-expression-in-the-small-intestine-mucosa-in-growing-pigs","status":"publish","type":"post","link":"http:\/\/prairieswine.com\/rsc\/electron-microscopy-finder-grids-link-quantitative-cytometry-with-high-resolution-confocal-imaging-in-the-study-of-tgf-%ce%b21-expression-in-the-small-intestine-mucosa-in-growing-pigs\/","title":{"rendered":"Electron microscopy finder-grids link quantitative cytometry with high resolution confocal imaging in the study of TGF-\u03b21 expression in the small intestine mucosa in growing pigs"},"content":{"rendered":"<p class=\"MsoNormal\" style=\"margin: 0in 0in 0pt; mso-layout-grid-align: none;\"><span style=\"font-family: Times New Roman; font-size: small;\">A new method is proposed linking the high-throughput quantitative population analysis with high-resolution imaging systems. Samples from piglet small intestine labelled against transforming growth factor \u03b21 (TGF-\u03b21) were overlayed with electron microscopy finder grids and scanned by SCAN^R scanning cytometer. From the tissue map generated by SCAN^R, regions of interest (ROI) with TGF-\u03b21-positive cells were selected and their coordinates stored from the sample overview. ROI were then located under the confocal microscope enabling detail visualization of the pattern of intracellular localization of the studied cytokine<\/span><\/p>\n<p class=\"MsoNormal\" style=\"margin: 0in 0in 0pt; mso-layout-grid-align: none;\">\u00a0<\/p>\n<p class=\"MsoNormal\" style=\"margin: 0in 0in 0pt; mso-layout-grid-align: none;\">\u00a0<\/p>\n<p>For more information the full article can be found at <a href=\"http:\/\/www.journals.elsevierhealth.com\/periodicals\/livsci\">http:\/\/www.journals.elsevierhealth.com\/periodicals\/livsci<\/a><\/p>\n<p class=\"MsoNormal\" style=\"margin: 0in 0in 0pt; mso-layout-grid-align: none;\"><span style=\"font-family: Times New Roman; font-size: small;\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A new method is proposed linking the high-throughput quantitative population analysis with high-resolution imaging systems. Samples from piglet small intestine labelled against transforming growth factor \u03b21 (TGF-\u03b21) were overlayed with electron microscopy finder grids and scanned by SCAN^R scanning cytometer. From the tissue map generated by SCAN^R, regions of interest (ROI) with TGF-\u03b21-positive cells were [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[930],"tags":[8882,21168,13816,2817,8345,20007,651,517,158,1374,111,8771,8791,10859,14,8348,126,33,16262,15936,9663,8346,1533,35,20911,1579,8347,11771],"class_list":["post-8817","post","type-post","status-publish","format-standard","hentry","category-production","tag-al","tag-anna-kotunia-zabielski","tag-ass","tag-cla","tag-electron-microscopy-finder-grids","tag-gh","tag-growing-pig","tag-growing-pigs","tag-growth","tag-growth-rate","tag-health","tag-ingredient-feed-mww","tag-map","tag-nat","tag-pig","tag-pig-intestine","tag-piglet","tag-pigs","tag-pl","tag-romuald-zabielski","tag-romuald-zabielski-2011","tag-scanning-cytometry","tag-size","tag-swine","tag-t","tag-test","tag-tgf-1","tag-www-prairieswine-com"],"_links":{"self":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/8817","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=8817"}],"version-history":[{"count":3,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/8817\/revisions"}],"predecessor-version":[{"id":10349,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/8817\/revisions\/10349"}],"wp:attachment":[{"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=8817"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=8817"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=8817"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}