If allowed to rely on visual, auditory, and olfactory cues from live stimulus birds, domestic fowl are able to discriminate between familiar and unfamiliar conspecifics. However, some wild fowl are forced to rely on non-visual cues to recognize their group mates. In the current study, domestic chickens were tested to see if they could discriminate between familiar and unfamiliar conspecifics in the absence of visual cues. Two preference experiments were conducted. In each test, two live hens (one familiar and one unfamiliar) were presented to the test hen. In experiment one, the test hen was separated by the stimulus hen by only a wire-mesh, allowing the test hen to receive visual, auditory, and olfactory cues from the stimulus hens. In experiment two, visual contact was prevented by using an opaque black cloth as a separator rather than the wire-mesh. The aggregation time with the familiar and unfamiliar bird was recorded as a measure of preference. In experiment one, hens showed significant discrimination between familiar and unfamiliar conspecifics, although their preference for the familiar or unfamiliar bird depended on the experimental series in which they were housed. Hens in experiment two, which had to rely on non-visual cues, did not appear able to discriminate between familiar and unfamiliar hens.