A multilevel nonlinear mixed-effects approach to model growth in pigs
Posted in: Production by admin on August 8, 2011 | No Comments
Growth functions have been used to predict market weight of pigs and maximize return over feed costs. This study was undertaken to compare 4 growth functions and methods of analyzing data, particularly one that considers nonlinear repeated measures. Data were collected from an experiment with 40 pigs maintained from birth to maturity and their BW measured weekly or every 2 wk up to 1,007 d. Gompertz, logistic, Bridges, and Lopez functions were fitted to the data and compared using information criteria. For each function, a multilevel nonlinear mixed effects model was employed because it allowed for estimation of all growth profiles simultaneously, and different sources of variation (i.e., sex, pig, and litter effects) were incorporated directly into the parameters. Furthermore, variance in-homogeneity and within-pig correlation were introduced to the functions. Inclusion of a variance of power function and a continuous autoregressive process of first order rendered a substantially improved fit to data for all 4 growth functions. The Lopez function provided the best fit to the data set and was used for characterizing mean growth curves for the 3 sexes (barrows, boars, and gilts). It was estimated that the maximum growth rate occurs at 117, 134, and 96 kg of BW for barrows, boars, and gilts, respectively. Hence, the gilts reached their maximum growth rate at an earlier stage in life compared with boars. Mature size of pigs varied systematically with sex and was estimated to be 466, 537, and 382 kg of BW for the barrows, boars, and gilts, respectively. These estimates are significantly affected by the duration of the experimental period, and it is recommended that future studies looking at estimating the mature size in animals are conducted long enough so that the BW visually stabilizes. Furthermore, studies should consider adding continuous autoregressive process when analyzing nonlinear mixed models with repeated measures.
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A multivariate nonlinear mixed effects method for analyzing energy partitioning in growing pigs
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Simultaneous equations have become increasingly popular for describing the effects of nutrition on the utilization of ME for protein (PD) and lipid deposition (LD) in animals. The study developed a multivariate nonlinear mixed effects (MNLME) framework and compared it with an alternative method for estimating parameters in simultaneous equations that described energy metabolism in growing pigs, and then proposed new PD and LD equations. The general statistical framework was implemented in the NLMIXED procedure in SAS. Alternative PD and LD equations were also developed, which assumed that the instantaneous response curve of an animal to varying energy supply followed the law of diminishing returns behavior. The Michaelis-Menten function was adopted to represent a biological relationship in which the affinity constant (k) represented the sensitivity of PD to ME above maintenance. The approach accommodated inclusion of a PD potential (PDPotential) concept. This was described by a Gompertz function, which was parameterized in terms of the maximum rate of PD (PDMax) and corresponding BW (BWPDMax) at that point. Metabolizable energy for LD was equated to the difference between ME intake and the sum of ME used for maintenance and PD. Metabolizable energy designated for PD and LD was used, with efficiencies kp and kf, respectively. The new equations were compared with the van Milgen and Noblet (1999) equations using 2 comprehensive data sets on energy metabolism in growing pigs. The 2 equation sets were evaluated using information criteria, which showed that the new equations performed best for data set II, whereas the reverse was true for the first. For the data set I population, estimates for kp and kf were 0.57 and 0.84, respectively. Maintenance was quantified as 1.10 MJ/d∙kg0.55. The animal variation in the parameter kp was estimated to be 6% CV. The animal variation in PDMax and kf was estimated to be 9 and 10% of the population estimates, respectively. It was conclude that application of the MNLME framework was superior to the multivariate nonlinear regression model because the MNLME method accounted for correlated errors associated with PD and LD measurements and could also include the random effect of animal. It is recommended that multivariate models used to quantify energy metabolism in growing pigs should account for animal variability and correlated measurement errors.
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Effect of virginiamycin on the apparent ileal digestibility of amino acids by growing pigs
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The objective of this experiment was to measure the influence of virginiamycin on the apparent ileal digestibility (AID) of AA by growing pigs. Fifteen barrows were surgically equipped with a T-cannula in the distal ileum and used in the experiment (initial BW = 35.0 kg). Animals were randomly allotted to 3 dietary treatments with 5 pigs per treatment during a 6-wk experiment. Dietary treatments included 1) a basal corn-soybean meal diet, 2) the basal diet supplemented with 11 mg/kg of virginiamycin, and 3) the basal diet supplemented with 22 mg/kg of virginiamycin. Pigs were fed their respective treatment diets during wk 2, 3, and 4, but during wk 1, 5, and 6, all pigs were fed the basal diet. Ileal samples were collected on d 6 and 7 of each week. Results showed that the AID of all indispensable AA, except Arg, His, and Ile, increased during wk 2, 3, and 4 compared with wk 1 in pigs fed the diet containing 11 mg/kg of virginiamycin. Pigs fed 22 mg/kg of virginiamycin during wk 2, 3, and 4 had increased AID of Trp and Val during these weeks compared with the AID in wk 1. However, the increased AID of AA did not carry over to wk 5 and 6, when virginiamycin was withdrawn from the diet, regardless of the inclusion rate. In pooled data from wk 2, 3, and 4, the AID of CP, the mean of all indispensable AA, and Ile, Leu, Met, Phe, Trp, and Val increased (linear) as virginiamycin was added to the diets, whereas a tendency for a linear or quadratic increase was observed for His, Lys, and Thr. These results indicate that addition of virginiamycin to corn-soybean meal diets fed to growing pigs increases the AID of AA, but this effect is not maintained after the removal of virginiamycin from the diet.
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Dietary effects of distillers dried grains with solubles on performance and milk composition of lactating sows
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A study was conducted to evaluate the dietary effect of adding increasing concentrations of distillers dried grains with solubles (DDGS) to cornand soybean meal-based sow lactation diets on sow and litter performance, energy and N digestibility, plasma urea N (PUN), and milk fat and protein concentrations. Mixed-parity sows [n = 307; 221 kg of BW, 4.54 parities, litter size of 10.6, and litter weight at birth (alive) of 15.14 kg] were assigned randomly to 1 of 5 dietary treatments: control (CON; corn-soybean meal); 10, 20, and 30% DDGS; and 30% DDGS high-protein (HP) diets. Sows were moved to farrowing rooms on d 109 of gestation and were fed the dietary treatments until weaning. Within each treatment group, feces and urine for energy and N digestibility analysis (from d 10 to 12 of lactation) and blood for PUN analysis and milk fat and protein concentrations (on d 0 and 19 of lactation) were collected from 6 randomly chosen parity 3 to 5 sows. There were no dietary effects of DDGS on ADFI of sows and sow backfat change. However, sows fed 30% DDGS HP lost more BW compared with sows fed CON. There were no dietary effects of DDGS on preweaning mortality of piglets, litter weight gain, and piglet ADG. Dietary treatments did not affect DE, ME, N retention, or N digestibility of the diets. There were no differences in the concentrations of fat and protein in milk at weaning (d 19) among dietary treatments. Sows fed 20 and 30% DDGS had less PUN at weaning (d 19) than sows fed CON and 30% DDGS HP. Inclusion of up to 30% DDGS in a lactation diet did not affect sow and litter performance, DE and ME contents of the diets, N retention and digestibility, and milk composition compared with sows fed a cornsoybean meal CON diet. It was concluded that addition of up to 30% DDGS in a lactation diet will support satisfactory sow and litter performance.
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Effect of polymorphisms in candidate genes on reproduction traits in Finnish pig populations
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Reproduction traits play an important role in economically viable piglet production and are closely related to the quality and length of the productive life of the sow. A increased removal rate of young sows is undesirable not only because of the associated financial penalties incurred, but also because of ethical concerns. Candidate genes and gene pathways have been identified for fertility in model species, and recent studies have provided evidence that polymorphisms within these genes are associated with reproduction traits in American Yorkshire/Large White and Landrace populations. In this study we evaluated the impact of single polymorphisms (n = 7) in 7 candidate genes on reproductive efficiency in Finnish Yorkshire (n = 280) and Landrace (n = 271) populations: IGFBP1, IGFBP2, IGFBP3, IGFBP5, CPTIA (carnitine O-palmitoyltransferase I), COX2 (PG-endoperoxide synthase 2, also known as cyclooxgenase-2), and SLC22A5 [organic cation/carnitine transporter 2 (solute carrier family member I), OCTN2]. In the Finnish Yorkshire population, only 4 of the analyzed markers were polymorphic. Significant effects on farrowing time were detected from the Yorkshire data, with polymorphisms within the genes CPT1A [a (allele substitution effect of allele A) = 2.97 d for age at first farrowing)], IGFBP3 (a = 0.54 d for farrowing interval of parities >1), and IGFBP5 (a = 3.22, 1.27, and 0.85 d for age at first farrowing and farrowing interval in the first and later parities, respectively). For the Landrace population, 6 markers were polymorphic, and significant effects were detected for traits affecting litter size. The polymorphism within the COX2 gene had an additive effect of 0.3 piglets for litter size in parities >1, and the IGFBP1 gene had an additive effect of 0.21, 0.26, and 0.11 piglets for litter size in the first parity, parities >1, and stillborn in parities >1, respectively. The additive effect of the SNP within the IGFBP2 gene was 0.16, 0.09, and 0.09 piglets for litter size in parities >1 and stillborn in the first and later parities, respectively. Finally, the IGFBP5 gene had an additive effect of 0.18, 0.07, and 0.07 piglets for litter size in the first parity, stillborn in parities >1, and mortality between farrowing and weaning in the first parity, respectively. These results support the suitability of the candidate gene approach for identification of markers to improve the reproductive performance of sows and to provide potential markers for marker-assisted selection.
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Effect of β-glucans contained in barley- and oat-based diets and exogenous enzyme supplementation on gastrointestinal fermentation of finisher pigs and subsequent manure odor and ammonia emissions
Posted in: Environment, Production by admin on August 5, 2011 | No Comments
The objective of the current experiment was to evaluate the influence of dietary cereal sources of β(1,3)(1,4)-d-glucan (β-glucan) and enzyme supplementation on indices of environmental pollution from finisher pigs. An experiment with a 2 × 2 factorial arrangement of treatments was initiated to investigate the effect of dietary source of β-glucan (barley vs. oats) and enzyme supplementation (no vs. yes) on nutrient digestibility, N utilization, intestinal fermentation, and manure odor and ammonia emissions from finisher boars (n = 4; BW = 73.9 kg; SD = 4.7). Sixteen boars were assigned to 1 of 4 dietary treatments (n = 4/treatment): 1) barley-based diet, 2) barleybased diet + exogenous enzyme, 3) oat-based diet, and 4) oat-based diet + enzyme. The enzyme supplement used contained endo-1,3(4)-β-glucanase and endo-1,4- β-xylanase. Experimental diets were formulated to contain similar concentrations of DE (13.5 MJ/kg) and digestible lysine (8.8 g/kg). Pigs offered oat-based diets had reduced digestibility of DM (0.795 vs. 0.849), OM (0.808 vs. 0.865), GE (0.806 vs. 0.845), and NDF (0.233 vs. 0.423) compared with those offered barley-based diets. Oat-based diets increased populations of Bifidobacterium spp. (7.26 vs. 6.38 log cfu/g of digesta) and Lactobacillus spp. (6.99 vs. 6.18 log cfu/g of digesta) in the proximal colon and decreased manure odor emissions [2,179.6 vs. 4,984.6 OuE/m3 (where OuE refers to European odor units)] compared with barleybased diets. There was an interaction between cereal type and enzyme inclusion on manure ammonia emissions from 0 to 96 h. Pigs offered barley- based diets containing an enzyme supplement had increased manure ammonia emissions compared with those offered unsupplemented barley-based diets. However, there was no effect of enzyme inclusion on oatbased diets. In conclusion, pigs offered oat-based diets harbored increased Bifidobacterium spp. and Lactobacillus spp. populations in the proximal colon and had decreased manure odor emissions compared with those offered barley-based diets. Enzyme inclusion had no effect on manure ammonia emissions from pigs offered oat-based diets.
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The nutritional value of expeller-pressed canola meal for grower-finisher pigs
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Expeller-pressed (EP) canola meal contains more residual oil than solvent-extracted canola meal and might be an attractive feedstuff for swine, but it has been poorly characterized. In Exp. 1, six ileal-cannulated barrows (36 kg of BW) were fed at 3× maintenance either a 44% EP canola meal diet or a N-free diet in a crossover design to measure energy and AA digestibility and calculate standardized ileal digestible (SID) AA and NE content, with 6 observations per diet. Each period consisted of a 5-d diet adaptation and a 2-d feces and 3-d digesta collection. The EP canola meal contained (% of DM) 38.5% CP, 13.3% ether extract, 2.42% Lys, 1.54% Thr, 0.62% Met, and 23.2 μmol/g of glucosinolates. Apparent total tract energy digestibility was 75.0% and the DE and predicted NE content were 3.77 and 2.55 Mcal/kg (in DM), respectively. The SID AA content (% of DM) was 1.77% Lys, 1.04% Thr, and 0.52% Met. In Exp. 2, a total of 1,100 pigs (25 kg of BW) housed in 50 pens were fed 5 dietary regimens with 0, 7.5, 15, and 22.5% or decreasing amounts (22.5, 15, 7.5, and 0%, respectively) of EP canola meal over 4 phases to validate performance and carcass characteristics. Diets were formulated to contain equal NE:SID Lys for each growth phase (g/Mcal; 4.04, d 0 to 25; 3.63, d 26 to 50; 3.23, d 51 to 77; 2.83, d 78 to 90). At slaughter, carcass characteristics were measured for all pigs, and jowl fat was sampled for 2 pigs per pen. For d 51 to 90, the 22.5% EP canola meal regimen was reduced to 18% (22.5/18%) because of decreased ADFI in phases 1 and 2. Overall (d 0 to 90), increasing dietary EP canola meal linearly decreased ADG and ADFI and linearly increased G:F. For 0 and 22.5/18% EP canola meal, respectively, ADG was 978 and 931 g/d, ADFI was 2.77 and 2.58 kg/d, and G:F was 0.366 and 0.378. Increasing dietary EP canola meal did not alter the carcass backfat thickness, loin depth, or jowl fat fatty acid profile. Pigs fed 22.5/18% EP canola meal reached slaughter weight 3 d after pigs fed 0% EP canola meal. In summary, EP canola meal provided adequate energy and AA; however, ADG was reduced by 3 g/d per 1% of EP canola meal inclusion, likely because of increased dietary glucosinolates. Thus, the amount of EP canola meal included in swine diets should be targeted to an expected growth performance and carcass quality. Finally, diets formulated to contain an equal NE and SID AA content did not entirely eliminate the risks for reduce growth performance associated with inclusion of an alternative feedstuff.
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Feed preferences and performance of nursery pigs fed diets containing various inclusion amounts and qualities of distillers coproducts and flavor
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We evaluated the preferences of nursery pigs for diets containing increasing distillers dried grains with solubles (DDGS), varying in color, or highprotein distillers dried grains (HP-DDG) and the effects of flavor supplementation on pig preference and growth performance. In Exp. 1 through 5, diet preference was determined in weanling pigs adjusted to a commercial diet for at least 10 d, and then housed individually for a 2-d double-choice preference test. In Exp. 1, a total of 60 pigs (11.6 kg of BW) were given a choice between a reference diet (0% DDGS) and test diets containing 0, 10, 20, or 30% DDGS. In Exp. 2, a total of 80 pigs (10.8 kg of BW) were given a choice between a reference diet (0% HP-DDG) and diets containing 0, 10, 20, or 30% HP-DDG. In Exp. 3, a total of 80 pigs (10.3 kg of BW) were given a choice between a reference diet (0% DDGS) and a diet containing 0%, 30% light, or 30% dark DDGS. In Exp. 4, a total of 80 pigs (11.2 kg of BW) were given a choice between a reference diet without DDGS and a diet containing either 0% DDGS, 10 or 20% light DDGS, or 10 or 20% dark DDGS. In Exp. 5, a total of 108 pigs (9.0 kg of BW) were given a choice between a reference diet (0% DDGS and no flavor) and a diet without or with flavor and containing 0, 10, or 20% DDGS. In Exp. 1 and 2, DDGS and HP-DDG, respectively, linearly decreased pig preference. In Exp. 3, dark DDGS were preferred compared with light DDGS. In Exp. 4, preferences were linearly reduced with DDGS inclusion, and dark DDGS tended to be preferred compared with light DDGS. In Exp. 5, DDGS reduced preference and flavor reduced preference regardless of DDGS level. In Exp. 6, a total of 192 pigs (6.7kg of BW) were fed starter 1 diets without or with flavor for 1 wk. Subsequently, pigs were fed starter 2 and 3 diets (2 wk each) containing 0, 10, or 20% DDGS while continuing to receive their respective flavor treatment. Flavor addition during the starter 1 phase increased ADFI, and DDGS inclusion tended to decrease ADG and decreased ADFI during the starter 2 phase. Volatile components in DDGS and HPDDG varied greatly depending on the source. Nursery pigs preferred a diet without DDGS or HP-DDG, and this appeared to be unrelated to color differences between sources. Knowledge of volatile compounds that enhance or suppress the palatability of feed may lead to further development of feed additives for masking relatively unpalatable, albeit cost-effective, ingredients.
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Results from six generations of selection for intramuscular fat in Duroc swine using real-time ultrasound. II. Genetic parameters and trends
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Design of breeding programs requires knowledge of variance components that exist for traits included in specific breeding goals and the genetic relationships that exist among traits of economic importance. A study was conducted to evaluate direct and correlated genetic responses to selection for intramuscular fat (IMF) and to estimate genetic parameters for economically important traits in Duroc swine. Forty gilts were purchased from US breeders and randomly mated for 2 generations to boars available in regional boar studs to develop a base population of 56 litters. Littermate pairs of gilts from this population were randomly assigned to a select line (SL) or control line (CL) and mated to the same boar to establish genetic ties between lines. In the SL, the top 10 boars and 75 gilts were selected based on IMF EBV obtained from a bivariate animal model that included IMF evaluated on the carcass and IMF predicted via ultrasound. One boar from each sire family and 50 to 60 gilts representing all sire families were randomly selected to maintain the CL. Carcass and ultrasound IMF were both moderately heritable (0.31 and 0.38, respectively). Moderate to high genetic relationships were estimated among carcass backfat and meat quality measures of IMF, Instron tenderness, and objective loin muscle color. Based on estimates obtained in this study, more desirable genetic merit for pH is associated with greater genetic value for loin color, tenderness, and sensory characteristics. Intramuscular fat measures obtained on the carcass and predicted using ultrasound technology were highly correlated (rg = 0.86 from a 12-trait analysis; rg = 0.90 from a 5-trait analysis). Estimated genetic relationships among IMF measures and other traits evaluated were generally consistent. Intramuscular fat measures were also genetically associated with Instron tenderness and flavor score in a desirable direction. Direct genetic response in IMF measures observed in the SL corresponded to a significant decrease in EBV for carcass loin muscle area (−0.90 cm2 per generation) and an increase in carcass backfat EBV (0.98 mm per generation). Selection for IMF has led to more desirable EBV for objective tenderness and has had an adverse effect on additive genetic merit for objective loin color.
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Determining the effect of lysine: calorie ratio on growth performance of ten- to twenty-kilogram of body weight nursery pigs of two different genotypes
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Four experiments were conducted to determine the effects of standardized ileal digestible (SID) Lys:calorie (Lys:Mcal) ratio on growth performance of 10- to 20-kg pigs of 2 different genotypes. Experiment 1 (360 pigs, average BW = 10.2 kg; source 1) and Exp. 2 (351 pigs; average BW = 9.3 kg; source 2), were both organized as a combination of 2 simultaneous experiments with the first set of diets consisting of 5 treatments with increasing SID Lys and the second set of diets consisting of 5 treatments with increasing energy density (Exp. 1: 9.9, 10.7, 11.5, 12.2, and 13.0 g/kg of Lys and 2.95, 3.09, 3.24, 3.38, and 3.52 Mcal/ kg of ME, respectively; Exp. 2: 11.1, 11.9, 12.6, 13.4, and 14.2 g/kg and 2.95, 3.10, 3.25, 3.40, and 3.55 Mcal of ME/kg, respectively). In Exp. 1, increasing dietary SID Lys increased (linear) ADG and G:F, and increasing dietary ME increased (quadratic) G:F. In Exp. 1 the optimal Lys:Mcal ratio was estimated to be at least 4.1 g of Lys/Mcal of ME based on G:F. In Exp. 2, increasing dietary SID Lys increased (linear) ADG and G:F. Increasing dietary ME increased (linear) G:F. Because of the linear responses in this experiment, optimal Lys:Mcal ratio was at least 4.0 g of Lys/Mcal of ME. In Exp. 3 (350 pigs; average BW = 9.4 kg; source 1) and Exp. 4 (350 pigs; average BW = 7.5 kg; source 2), Lys:Mcal ratios in Exp. 1 and 2 were compared by titrating Lys at 2 energy levels. Pigs were fed diets with 2.95 or 3.29 Mcal/kg of ME with SID Lys:Mcal ratios of 3.1 to 4.1 g/Mcal of ME (Exp. 3) and 3.5 to 4.5 g/Mcal of ME (Exp. 4). In Exp. 3, there was an ME × Lys:Mcal ratio interaction for ADG. The greatest ADG was a Lys:Mcal ratio of 3.60 for pigs fed low ME and a ratio of 3.35 for pigs fed high ME. Gain:feed ratio increased with increased ME concentration and as Lys:Mcal ratio increased (quadratic); the best G:F was observed at 3.67 g of Lys/Mcal of ME. In Exp. 4, there was a tendency for ME × Lys:Mcal ratio interaction for G:F. The greatest G:F was achieved with a Lys: Mcal ratio of at least 4.50 for pigs fed low ME and a ratio of 4.29 for pigs fed high ME. Results (i.e., lack of ADG response to high energy density) suggest pigs reared in this environment were not in energy dependent growth phases. These pigs needed approximately 11 g/d of SID Lys (19 g of Lys/kg of gain) to optimize ADG and G:F. Based on these results, optimal Lys:Mcal ratio may differ depending on daily caloric intake of the pig.
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