In order to evaluate skeletal quality and eggshell parameters, a study was conducted on molted hens at the end of the second laying cycle. Sixty hens were allocated to two control and four molting treatments: full-fed control 1 (82 weeks old), full-fed control 2 (122 weeks old), feed withdrawal, 100 % alfalfa, 90 % alfalfa/10 % layer ration, and 70 % alfalfa/30 % layer ration. Hens were euthanized at the end of the second laying cycle by CO2. The femur and tibia were collected. There were no differences in bone parameters among the two control treatment hens, or among the different molting treatment hens. Egg weights were heavier but percentage shell and egg production were lower among ‘full-fed control 2’ birds compared with ‘full-fed control 1’ birds. The ‘feed-withdrawal’ hens and ’90 % alfalfa/10 % layer ration’ hens had heavier shell weights than the ‘100 % alfalfa’ hens. Overall bone parameters were found to be negatively correlated with eggshell parameters. Bone parameters were highly correlated with each other. Shell weight, percentage shell, and shell thickness were positively correlated with each other while egg weight was negatively correlated with percentage shell. Results of the study indicated that the age of the hens and molting dietary treatments influence egg parameters, and eggshell formation is closely related to bone metabolism in laying hens.
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