This study examined transport conditions and behavioural and physiological responses of pigs transported in summer and winter, in both Eastern and Western Canada. Data were collected during all stages of transport, including loading, transport, unloading and lairage (waiting) at the abattoir. Measures included truck temperature, pig behaviour, core body temperature, heart rate, blood measures of stress and meat quality. The presence of steep internal ramps in pot belly trailers had a significant impact on the handling behaviour and heart rate of pigs at loading and unloading. Significant variation in temperatures was found between compartments within pot belly trailers during transport, both in summer and winter. The combination of handling conditions and truck temperatures had a significant impact on pork quality. In the winter trials a higher incidence of dark firm and dry (DFD) or moderate DFD pork was found, especially in the west where pigs experienced longer transport times. In the summer trials, a higher incidence of pale, soft and exudative (PSE) or moderate PSE pork was found.