This study focused on the movement of bacteria to receiving tile drains following swine manure application. Specifically, the impacts of different manure application regimes on fecal coliform (FC), Enterococcus (EN), and Escherichia coli (EC) densities in subsurface tile drain water were examined for three years. Results of this study suggest that manure broadcast onto frozen ground may lead to significantly elevated EN and EC levels in tile water in similar environments, especially when applied in excess of crop nutrient requirements.