Spontaneous early and mid-gestation fetal losses occur in swine. At both stages, endometrial lymphocytes associated with smaller, paler conceptuses have fewer pro-angiogenic and more pro-inflammatory cytokine transcripts compared with robust conceptuses. We hypothesized that similar differences occur in conceptus-associated dendritic cells (DCs). Using laser capturemicrodissection, dendritic cell-specific intercellular adhesion molecule-grabbing non-integrin (DC-SIGN)+ cells were isolated from attachment sites of healthy and arresting conceptuses at gestation day (gd)20 and 50. DC-SIGN+ cells were screened using real-time PCR for vascular endothelial cell growth factor (Vegf), its receptors, semaphorins (Sema) and plexins (Plxn), and for toll-like receptor (Tlr) transcripts to address potential activation pathways. Homogenized endometrial and trophoblast biopsies were quantified for type 1\/type 2 cytokine transcripts\/proteins. DC-SIGN+ cells from healthy and arresting conceptuses had more Vegf transcripts at early than mid gestation whereas transcripts for Vegfr1 and Vegfr2 were stable. In gd20 arresting site DC-SIGN+ cells, Neuropilin-2 transcripts were elevated, whereas at gd50 arresting sites, Plxn-A2 increased and Sema3A transcripts were lost. Tlr-1, Tlr-4 and Tlr-6 transcript abundance was independent of conceptus health. At gd20, type 1 cytokines were prevalent, whereas at gd50 type 2 cytokines predominated in endometrium and trophoblast. Thus, gestational features, characteristic of haemochorial placentation, are present in species with distinctly different placentation.<\/p>\n","protected":false},"excerpt":{"rendered":"
Spontaneous early and mid-gestation fetal losses occur in swine. At both stages, endometrial lymphocytes associated with smaller, paler conceptuses have fewer pro-angiogenic and more pro-inflammatory cytokine transcripts compared with robust conceptuses. We hypothesized that similar differences occur in conceptus-associated dendritic cells (DCs). Using laser capturemicrodissection, dendritic cell-specific intercellular adhesion molecule-grabbing non-integrin (DC-SIGN)+ cells were isolated […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18120,19,930],"tags":[102,35],"class_list":["post-14821","post","type-post","status-publish","format-standard","hentry","category-ontario-pork","category-pork-insight-articles","category-production","tag-gestation","tag-swine"],"_links":{"self":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14821","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=14821"}],"version-history":[{"count":1,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14821\/revisions"}],"predecessor-version":[{"id":14822,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/14821\/revisions\/14822"}],"wp:attachment":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=14821"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=14821"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=14821"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}