{"id":15057,"date":"2008-03-20T00:00:38","date_gmt":"2008-03-20T06:00:38","guid":{"rendered":"http:\/\/prairieswine.com\/rsc\/?p=15057"},"modified":"2013-08-21T14:58:06","modified_gmt":"2013-08-21T20:58:06","slug":"adverse-effects-induced-by-short-hairpin-rna-expression-in-porcine-fetal-fibroblasts","status":"publish","type":"post","link":"https:\/\/prairieswine.com\/rsc\/adverse-effects-induced-by-short-hairpin-rna-expression-in-porcine-fetal-fibroblasts\/","title":{"rendered":"Adverse effects induced by short hairpin RNA expression in porcine fetal fibroblasts"},"content":{"rendered":"<p>RNA interference is a recent, gene silencing technique that could be extremely valuable in studying gene function, treating diseases, and developing novel animal models for human diseases. Here, we investigated the feasibility of applying shRNA-mediated RNA interference in fetal fibroblasts for silencing of the myostatin gene and investigate adverse effects of RNAi. We report that up to 97% silencing of myostatin mRNA was achieved using shRNA constructs in transiently and stably transfected fetal fibroblasts (p &lt; 0.05). At the same time we also demonstrate that high level of shRNA expression resulted in 10- to 1000-fold induction of interferon responsive genes (OAS1, IFN-b) (p &lt; 0.05). In addition we also report novel adverse effect of shRNA expression in stably transfected cells\u2014interference with microRNA processing\/ transport which led to 500-fold increase in the level of miR21 precursors (p &lt; 0.05). Reduction of these side effects will be essential to obtain long term stable RNAi silencing.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>RNA interference is a recent, gene silencing technique that could be extremely valuable in studying gene function, treating diseases, and developing novel animal models for human diseases. Here, we investigated the feasibility of applying shRNA-mediated RNA interference in fetal fibroblasts for silencing of the myostatin gene and investigate adverse effects of RNAi. We report that [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18120,19],"tags":[640,5845,91],"class_list":["post-15057","post","type-post","status-publish","format-standard","hentry","category-ontario-pork","category-pork-insight-articles","tag-animal","tag-diseases","tag-transport"],"_links":{"self":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15057","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=15057"}],"version-history":[{"count":1,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15057\/revisions"}],"predecessor-version":[{"id":15059,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15057\/revisions\/15059"}],"wp:attachment":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=15057"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=15057"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=15057"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}