Recent studies have suggested that leptin plays an important role in reproduction. Ob-R is expressed in the murine embryo, and is suggested\u00a0to play a role in embryo development, although contradictory results have been reported. In the present study, Ob-R expression was observed\u00a0both at themRNAand protein levels in porcine early embryos.We have also demonstrated that leptin is produced in the porcine oviduct, making\u00a0it spatially available to interact with its receptor during preimplantation development. When included at 10 ng\/ml in embryo culture medium,\u00a0leptin significantly increased the proportion of cleaved embryos (P < 0.01). At day 7 of in vitro culture, leptin at 10 and 100 ng\/ml increased\u00a0the proportion of embryos reaching the blastocyst stage (P < 0.01). We have previously observed that leptin increases oocyte maturation in\u00a0vitro, and here we report that inclusion of leptin in both IVM and embryo culture medium further increased blastocyst development (P <0.05), compared to when leptin was included in the embryo culture alone, suggesting leptin has a synergistic role on both oocyte maturation\u00a0and preimplantation embryo development. \u00a0The study suggest that leptin plays\u00a0a positive role in the development of porcine preimplantation\u00a0embryos. In addition to adding to knowledge on regulation of\u00a0early embryo development, these results may also have clinical\u00a0applications. In the pig, the current success rate of IVF\u00a0and nuclear transfer techniques remains low. The finding that\u00a0the inclusion of leptin in IVM and embryo culture mediums\u00a0enhances early development suggests that leptin may aid in\u00a0the optimization of these techniques, possibly leading to improved\u00a0success rates.<\/p>\n","protected":false},"excerpt":{"rendered":"
Recent studies have suggested that leptin plays an important role in reproduction. Ob-R is expressed in the murine embryo, and is suggested\u00a0to play a role in embryo development, although contradictory results have been reported. In the present study, Ob-R expression was observed\u00a0both at themRNAand protein levels in porcine early embryos.We have also demonstrated that leptin […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18120,19],"tags":[14,816],"class_list":["post-15096","post","type-post","status-publish","format-standard","hentry","category-ontario-pork","category-pork-insight-articles","tag-pig","tag-protein-level"],"_links":{"self":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15096","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/comments?post=15096"}],"version-history":[{"count":1,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15096\/revisions"}],"predecessor-version":[{"id":15098,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/posts\/15096\/revisions\/15098"}],"wp:attachment":[{"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/media?parent=15096"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/categories?post=15096"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/prairieswine.com\/rsc\/wp-json\/wp\/v2\/tags?post=15096"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}