This study focused on the potential bacteria and nutrient contanimation of subsurface agricultural drainage with differing manure application rates on different types of tillage practices. Manure was surface applied to conventional and no-till practices at rates of 2.8 cm and 8.3 cm. The subsurface drainage water was analyzed for volume, pathogenic bacteria and nutrient concentrations. The results indictate higher manure applicaton rates produce higher levels of nutrient and bacteria containimation within 1 hour of application. The conventional tillage operation seemed to have a lower level of contaimination, resulting from reduced macropore flow from the tillage operation.