In 2003, legislation was passed in Manitoba that was designed to increase the production and utilization of Manitoba produced ethanol. The Biofuels Act sets in place requirements for the sale of ethanol-blended gasoline by 2007. In order to meet the increased demand for locally produced ethanol, consideration must be given to the effective utilization of the co-products from this industry, as this has proven to be a key determinant of the economic competitiveness of the process. The process of ethanol production results in a co-product, dried distillers’ grains with solubles (DDGS) that has potential as an ingredient for livestock feed. In North America, corn has been used as the main cereal grain in ethanol production and the resulting DDGS have been extensively evaluated as a feedstuff for swine. However, the feedstock generally considered for the Manitoba ethanol industry is wheat. The effective utilization of wheat based DDGS requires a complete appraisal of its nutritive value. In this first report, we will characterize the chemical composition of the DDGS produced from a local ethanol manufacturer. A subsequent report will provide more information as to the utilization of the product by swine. Samples of DDGS were obtained from the Husky Mohawk ethanol plant at Minnedosa, Manitoba. All samples were taken from different fermentation batches. In all, three separate samples of wheat DDGS (samples #1, 2, and 3) and one sample of a mixed corn/wheat DDGS were collected and analyzed for their contents of key nutrients, including crude protein, energy, fibre fractions, and mineral components. In summary, with respect to the nutrients measured, the wheat-based DDGS had a nutrient profile that was similar to that of canola meal, but contained higher amounts of crude protein and lower levels of calculated digestible energy than the corn DDGS. Soybean meal had higher levels of both protein and digestible energy than the wheat-based DDGS. The comparison of the nutrient profile of wheat-based DDGS to that of other plant-based protein sources is important, as this is the likely class in which this product will compete in least cost formulations. While the nutrient profile is critical for decision-making processes, a more accurate assessment of the value of the wheat-based DDGS requires their assessment during feeding studies with pigs.