The present paper describes the development of a methodology for rapid and effective simultaneous extraction of boar taint compounds from pig fat tissue. The applicability of a single LC–MSn method as a specific detection method for both indolic compounds and a steroidic compound was evaluated. The results showed that the simultaneous determination of ID, SK and AEON in back fat matrix was enabled combining minimised sample handling with a high specific mass spectrometric detection technique. However, as simultaneous analysis of structurally different indolic compounds and a steroid was assessed, future research will specifically address improvement of the extraction procedure.