Milk yield per cow has increased dramatically during past decades in many countries. Several studies have shown that a more frequent milking will increase the milk yield. Besides a possible economic benefit of a higher milking frequency, there may be other advantages to the individual high-producing cow, such as increased lying comfort. Cows with filled udders may have greater difficulty in performing certain movements, such as lying down and getting up. The objective of this study was to investigate if cows milked twice per day have more difficulty lying down and getting up and spend less time lying than cows milked three times per day. Seventeen cows of the Swedish Red and White Cattle Breed were studied, seven of which were milked twice daily (2M) and ten were milked three times (3M) daily. They were kept in individual cubicles, that were closed in the rear end with a rope. They had free access to a mixture of silage, hay and concentrate. The individual cows were video-recorded for 24 h every fourth week, starting four weeks after calving for four times. The 2M cows stood significantly longer than the 3M cows during the 4 h before morning milking. The 2M cows also had a tendency for longer duration of standing rumination as well as significantly more bouts of standing rumination during these hours than 3M cows. The cows in the 3M group spent less time on the getting up movement during the 4 h before morning milking. The distribution of the lying bouts during these 4 h differed significantly between the groups where the 3M cows had fewer lying bouts shorter than 15 min and more lying bouts longer than 90 min. The results of this study indicate that milking three times a day contributes to increased comfort in high-producing dairy cows.