Experimental and numerical modeling were conducted to determine the dust concentration distribution in a simulated mechanically ventilated swine nursery room under non-isothermal conditions. Effects on dust generation rate, temperature difference between air supply and inside air, dust source location, and presence of obstructions on dust concentrations were also evaluated. Resulst from the numeric modelling agreed with the measured values indicated that the dust source location significantly influenced the dust concentration distribution; dust concentration was higher at or near the source. However, the effect of temperature difference beyween supply air and inside air, dust generation rate and the presence of the mick pigs on dust concentration distribution were not statistically significant.