The effect of repeated handling and the use of a mask to cover their eyes on responses during restraint were investigated. Two experiments were conducted. During the first experiment, 18 cows were habituated to being restrained in a squeeze chute for three minutes. Blood samples were taken to determine cortisol levels. Habituation was indicated by a decrease in cortisol concentration over the test period. Six months later, the second experiment was conducted. Cows were randomly assigned to two groups, alternating the mask over test days. Cows were restrained as in the first experiment. At the beginning and end of each period of restraint, heart and respiratory rates were measured. A blood sample was taken at the end of the restraint. Respiratory rates, heart rates and cortisol concentrations were lower for masked cows than unmasked cows. Masked cows tended to be calmer than unmasked cows. The order of entrance was consistent over test days. In conclusion, Brahman cattle habituate to repeated experience in the squeeze chute, are calmer during the process when wearing a mask, and establish a consistent entrance order into the chute.