There are multiple stressors affecting the welfare of broiler chickens during transport. In this study, a preference test was used to determine which stressors birds would avoid if given the choice. The experiment was conduced in a choice-chamber, which had four outer compartments connected to a central zone. Each compartment had a different environment: no applied stressors, thermal stressor, vibrational stressor, or vibrational/thermal stressors. The birds were trained in the apparatus and then tested to determine which compartment they preferred. Training enabled the birds to become used to the environment in each compartment. Each compartment had a distinctive coloured wall so the birds could easily distinguish among the compartments. A feeding station was located at the back of each chamber. Prior to test days, the birds were fasted, so they would be motivated to enter a compartment to find food. The results indicated that the vibration stressor was significantly avoided, but the thermal stressor was not and there was no interaction. Individual birds were found to differ significantly. The avoidance of vibration was expected, but the lack of avoidance of the thermal stressor was not expected. Either the birds could not associate the delayed heat stress with the compartment, or they just did not find it aversive. Studying the effect of combinations of stressors is rare. Using a preference method enables greater insight into the birds perspective.