Recent concerns regarding antibiotic resistance and the use of probiotic agents in livestock has resulted in a demand for alternative strategies to improve animal production and health without the need for antibiotics. Several studies have been conducted to evaluate the effects of BioPlus 2B® supplement, which contains Bacillus subtilis and Bacillus licheniformis spores, on pigs, and the results of these studies have shown improved sow and piglet performance. Therefore, this experiment was conducted to assess the effects of supplementation of the diets of growing pigs with BioPlus 2B®. Specifically, the effects on growth performance, nutrient digestibility and noxious gas emission from pig slurry were evaluated. The results of this study indicate that supplementation of the diets of grower pigs with BioPlus 2B® did not significantly improve the growth performance. However, there was an effective reduction in the slurry NH3
emission in response to treatment with BioPlus 2B®. The underlying mechanisms by which this reduction occurred should be further assessed by evaluating the community structure of intestinal and fecal bacteria, which may better explain the relationship between dietary BioPlus 2B® and native bacterial populations.

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